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(TRIS, THAM, Tromethamine, Amino-2-(hydroxymethyl)-1,3-propanediol)

C4H11NO3 • Mr 121.1 • CAS [77-86-1]

Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.

With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications:

 Assays in molecular biology and cytology

 Extraction of proteins or nucleic acids from cells

 In situ hybridisation procedures

 Sample and running buffers for SDS-PAGE

 TAE- and TBE buffers for agarose gel electrophoresis

 Transfer buffer for Western Blotting

 Washing buffers for immunoassays

Assay (titr.)

A 1 cm/40 % in water

290 nm

pH (5 % in water)

Heavy metals (Pb)

min. 99.0 %

max. 0.2

10.0 - 11.5

max. 10 ppm

EINECS: 201-064-4 • WGK: 2L • HS: 29221900

Storage Temperature: +15 °C to +30 °C